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Effects of CBMN-CP on myocardial repair. a) Masson's trichrome staining of LV tissue for collagen (blue) and muscle (red) in the sham, MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of the LV wall thickness (b) and infarcted area (c) (n = 4). d) Representative immunofluorescence staining of the infarct region for cardiac-specific proteins of <t>α-actinin</t> (green), connexin 43 (Cx43) (red), and nuclei (blue). e, f) Fluorescence intensity statistics of α-actinin (e) and Cx-43 (f) based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of CBMN-CP on myocardial repair. a) Masson's trichrome staining of LV tissue for collagen (blue) and muscle (red) in the sham, MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of the LV wall thickness (b) and infarcted area (c) (n = 4). d) Representative immunofluorescence staining of the infarct region for cardiac-specific proteins of α-actinin (green), connexin 43 (Cx43) (red), and nuclei (blue). e, f) Fluorescence intensity statistics of α-actinin (e) and Cx-43 (f) based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Bioactive Materials

Article Title: A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium

doi: 10.1016/j.bioactmat.2025.10.045

Figure Lengend Snippet: Effects of CBMN-CP on myocardial repair. a) Masson's trichrome staining of LV tissue for collagen (blue) and muscle (red) in the sham, MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of the LV wall thickness (b) and infarcted area (c) (n = 4). d) Representative immunofluorescence staining of the infarct region for cardiac-specific proteins of α-actinin (green), connexin 43 (Cx43) (red), and nuclei (blue). e, f) Fluorescence intensity statistics of α-actinin (e) and Cx-43 (f) based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: After blocking for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies: Connexin 43 (BOSTER, BA1727, 1:1000, China), α-actinin (Peroteintech, 66895-1-ig, 1:25000, China) and BAX (BIOSS, bsm-33279m, 1:1200, China).

Techniques: Staining, Immunofluorescence, Fluorescence